pai1  (Santa Cruz Biotechnology)

Journal: Cancer Genomics & Proteomics

Article Title: Suppressing Expression of SERPINE1/PAI1 Through Activation of GPER1 Reduces Progression of Vulvar Carcinoma

doi: 10.21873/cgp.20473

Figure Lengend Snippet: A-D: Plasminogen activator inhibitor 1 (PAI1) expression is impacted by treating A431 and CAL39 cells with G-coupled estrogen receptor 1 (GPER1) agonist G1 and antagonist G36 (quantification via western blot). Expression of PAI1 in A431 (A, B) and CAL39 (C, D) cells treated with GPER1 agonist G1 (A, C) and GPER1 antagonist G36 (B, D) relative to ethanol treated control cells. Protein isolation was performed 72 h after treatment. E-F: Cell proliferation in vulvar squamous cell carcinoma (VSCC) cell lines is impacted by treatment with GPER agonist G1. A431 (E) and CAL39 (F) cells treated with ethanol control, GPER1 agonist G1 and GPER1 antagonist G36. Results measured relative to ethanol control after 4h in A431 and 10h in CAL39 cell line. Ordinary one-way ANOVA with Dunnett’s multiple comparisons test, mean with standard error of the mean (SEM); (A-C) n=3, (E) n=4, (F) n=11; **p<0.01 and ****p<0.0001.

Article Snippet: Primary antibodies targeted PAI1 at a 1:1,000 dilution (13801-1-AP, Proteintech, Planegg-Martinsried, Germany) and GAPDH at 1:2,000 dilution (5174S, Cell Signaling, Danvers, MA, USA).

Techniques: Expressing, Western Blot, Control, Isolation

Journal: Cancer Genomics & Proteomics

Article Title: Suppressing Expression of SERPINE1/PAI1 Through Activation of GPER1 Reduces Progression of Vulvar Carcinoma

doi: 10.21873/cgp.20473

Figure Lengend Snippet: Successful knockdown of PAI1 is confirmed by western blot (A, B) and the impact of SERPINE1/PAI1 knockdown on cell proliferation in A431 and CAL39 cells is shown (C, D). Relative gene expression of PAI1 in SERPINE1/PAI1 knockdown group compared to siRNA control in A431 (A) and CAL39 (B) cells 48 h after transfection. Cell viability in SERPINE1/PAI1 knockdown and siRNA control cells, in the A431 (C) and CAL39 (D) cell lines. Results measured relative to siRNA control after 10h in both cell lines. Unpaired t-test, two tailed, mean with standard error of the mean (SEM); (A, B) n=2, (C, D) n=9; *p<0.05, **p<0.01 and ***p<0.001.

Article Snippet: Primary antibodies targeted PAI1 at a 1:1,000 dilution (13801-1-AP, Proteintech, Planegg-Martinsried, Germany) and GAPDH at 1:2,000 dilution (5174S, Cell Signaling, Danvers, MA, USA).

Techniques: Knockdown, Western Blot, Gene Expression, Control, Transfection, Two Tailed Test

Journal: Cancer Genomics & Proteomics

Article Title: Suppressing Expression of SERPINE1/PAI1 Through Activation of GPER1 Reduces Progression of Vulvar Carcinoma

doi: 10.21873/cgp.20473

Figure Lengend Snippet: Migration in A431 vulvar SCC cells is reduced in SERPINE1/PAI1 knockdown cells. Gap closure in A431 (A, B) and CAL39 (C, D) cell lines after a successful SERPINE1/PAI1 knockdown compared with siRNA control. (B, D) Photos of the gaps in knockdown and control at 10h intervals; magnification×4. Ordinary one-way ANOVA with Tukey’s multiple comparisons test, mean with standard error of the mean (SEM); n=6; *p<0.05. The asterisks refer to significant differences between the corresponding graph line and the ethanol control.

Article Snippet: Primary antibodies targeted PAI1 at a 1:1,000 dilution (13801-1-AP, Proteintech, Planegg-Martinsried, Germany) and GAPDH at 1:2,000 dilution (5174S, Cell Signaling, Danvers, MA, USA).

Techniques: Migration, Knockdown, Control

Journal: Cancer Genomics & Proteomics

Article Title: Suppressing Expression of SERPINE1/PAI1 Through Activation of GPER1 Reduces Progression of Vulvar Carcinoma

doi: 10.21873/cgp.20473

Figure Lengend Snippet: (A, B) Tumor sphere formation and size are not significantly impacted in the A431 cell line in SERPINE1/PAI1 knockdown cells. 15 days after transfection, A431 tumor sphere formation (A) and size of spheres (B) are shown, relative to siRNA controls. (C-F) Colony size is reduced after SERPINE1/PAI1 knockdown in VSCC. Colony formation relative to siRNA control in A431 (C, D) and CAL39 cells (E, F). Number of colonies formed in A431 (C) and CAL39 (E) cell lines. Colony size in A431 (D) and CAL39 (F) cell lines compared to siRNA control. Unpaired t-test, two tailed, mean with standard error of the mean (SEM); (A, C) n=3, (C-F) n=6; *p<0.05.

Article Snippet: Primary antibodies targeted PAI1 at a 1:1,000 dilution (13801-1-AP, Proteintech, Planegg-Martinsried, Germany) and GAPDH at 1:2,000 dilution (5174S, Cell Signaling, Danvers, MA, USA).

Techniques: Knockdown, Transfection, Control, Two Tailed Test